diff options
Diffstat (limited to 'sci-biology/abyss/files/abyss-1.3.6-samtoafg.patch')
| -rw-r--r-- | sci-biology/abyss/files/abyss-1.3.6-samtoafg.patch | 40 |
1 files changed, 40 insertions, 0 deletions
diff --git a/sci-biology/abyss/files/abyss-1.3.6-samtoafg.patch b/sci-biology/abyss/files/abyss-1.3.6-samtoafg.patch new file mode 100644 index 000000000000..cb7dfb08a84d --- /dev/null +++ b/sci-biology/abyss/files/abyss-1.3.6-samtoafg.patch @@ -0,0 +1,40 @@ +Hello, + +I see Shaun Jackmans' (abyss-)sam2afg script is available within the +Amos source repository. I find this very useful for coercing output from +assemblers which which don't track read location into amos by remapping +reads against the assembly and converting to afg - not ideal but better +than nothing when you don't have the 'real' read locations to work with. + +This fails, however when reads are aligned with 'bwa mem', which can +output multi-part alignments. sam2afg checks for reuse of the same read +id (presumably to prevent the generation of non-unique eid values), +consequently encountering multiple alignments for a read causes it to die. + +The following one-line patch allows sam2afg to skip these secondary +alignments present in 'bwa mem' output, provided bwa mem has been run +with the '-M' argument which sets the SAM 'secondary alignment' flag on +the alignments in question. + +Hopefully this will also be of use to others... + +Best Regards, +James + +-- +Dr. James Abbott +Lead Bioinformatician +Bioinformatics Support Service +Imperial College, London + + +--- abyss-1.3.6/bin/abyss-samtoafg.ori 2015-05-23 23:43:46.797747928 +0200 ++++ abyss-1.3.6/bin/abyss-samtoafg 2015-05-23 23:44:09.227747743 +0200 +@@ -105,6 +105,7 @@ + die unless defined $qqual; + + $tstart--; # convert to zero-based coordinate ++ next if $flag & 0x100; # secondary alignment + $qid .= "/1" if $flag & 0x40; #FREAD1 + $qid .= "/2" if $flag & 0x80; #FREAD2 + |